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Iranian Journal of Veterinary Clinical Sciences
Evaluation of clove, Tobacco extract and Tricaine methane sulfonate sedation on some organs of common carp


 submission: - | acception: 10/02/2021 | publication: 20/09/2021

DOI 

Authors
behnaz shahryari zadeh1*, rahim peyghan2, annahita rezaie3

1-،shahriari_b@yahoo.com

2-،bn.shahriari@yahoo.com

3-،tabestanbarani@chmail.ir



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Abstract

In this study sedative effect of Clove extract and Tobacco extract and tricaine methane sulfonate (MS۲۲۲) on some organs of common carp (liver and gills) was studied. ۲۱۰ common carp were divided to ۷ groups (۲ Clove extract ۲۰ mg/l and ۴۰ mg/l, ۲ Tobacco extract ۴۰ mg/l and ۸۰ mg/l and ۲ tricaine methane sulfonate (MS۲۲۲) ۲۰ mg/l and ۴۰ mg/l for ۲ days and one group considered as control of study). Each group had ۱۰ fish with ۳ replicates. In days ۴ and ۶ after sedation ۳ fish from each treatment were randomly selected and sampling was carried from the liver and gill. After fixation with ۱۰% Formalin and routine procedures were performed in histopathological lab and Preparation of histopathological sections and staining sections, slides were examined. Histopathological examination revealed necrosis of lamella epithelium in Tobacco extract ۸۰ mg/l group. In other groups of clove extract and methane sulfonate trichinae (MS۲۲۲) no changes depend on this research were not seen in the liver and gills of fish. According to the microscopic results, clove extract has less pathological effects in comparison with Tobacco extract. More Histopathological research is definitely needed on the use of tobacco extract and clove extract in Aquaculture.




Keywords

Clove  Tobacco  Sedation  Histopathology. 



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