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Iranian Journal of Veterinary Clinical Sciences
Study of serum Cytokine IL-6 changes one week before and after parturition in ewe


 submission: - | acception: 02/07/2019 | publication: 05/07/2020

DOI 

Authors
Hadi Binandeh1*, Abdolnaser Mohebbi2, Farid Barati3, Naser Shams4

1-،hdbinandeh69@gmail.com

2-،ab-mohebi@vet.sku.ac.ir

3-،frdbrtir@gmail.com

4-،drn_shams@yahoo.com



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Abstract

Since the the natural delivery mechanism is related to the immunologic process, it is possible that level of different Inflammatory factors involved in immune responses (IL-۶ as Pro-Inflammatory Cytokine) may change in the near-parturition time (before and after parturition). The aim of this study is evulation of IL-۶ level change in the ewe, one week before until one week after Parturition. For this purpose a total ۴ pregnant ewes in the second term of their conception, are selected. Preservation and feeding was same for all of them. Blood sampling were taken from Jugular vein by means of nonobjective and vacuum pipe (۵-۷cc each ۲۴h, one week before parturition until one week after it). The blood samples for serum separation, were transferred to laboratory and after serum separation, for prevention of otolysis and destruction of immune chains were put in -۷۰ċ. IL-۶ was measured useing of specific kit and by Elisa test (Enzyme-linked immunodeficient assay). Results were showed that in the different days before and after parturition the level of IL-۶ has slightly changed that is insignificant (P = ۰.۷۶۲۵). In total result showed that serum level of IL-۶ in different times of parturition doesn’t affect.




Keywords

Key word: Parturition  Serum  IL ۶  Ewe 



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